HLA-Cw allele analysis by PCR-restriction fragment length polymorphism: study of known and additional alleles.
نویسندگان
چکیده
منابع مشابه
HLA-Cw allele analysis by PCR-restriction fragment length polymorphism: study of known and additional alleles.
We describe a technique for HLA-Cw genotyping by digestion of PCR-amplified genes with restriction endonucleases. Locus-specific primers selectively amplified HLA-Cw sequences from exon 2 in a single PCR that avoided coamplification of other classical and nonclassical class I genes. Amplified DNAs were digested with selected enzymes. Sixty-three homozygous cell lines from International Histocom...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.19.8803